Acti-stain™ 555 phalloidin from Cytoskeleton, Inc.

Product Specs
ItemActi-stain™ 555 phalloidin
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPHDH1-A
Quantity1 x 500 µl (300 slides)
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clustersElifeDecember 12, 2023Anshuman Bhanja, Margaret K. Seeley-Fallen, Michelle Lazzaro, Arpita Upadhyaya, Wenxia Song(1:200) Other,Acti-stain-488 Phalloidin,Cytoskeleton,PHDG1-A,IF (200 nM) Other,Acti-stain-555 Phalloidin,Cytoskeleton,PHDH1-A,IF (200 nM) Antibody,Rabbit IgG anti-mouse NMIIA antibody (rabbit polyclonal),Abcam,ab75590,IF (1:100) Antibody,Rabbit

(2) Polymer-Functionalized Mitochondrial Transplantation to Fibroblasts Counteracts a Pro-Fibrotic PhenotypeInternational journal of molecular sciencesJune 30, 2023Baudo G, Wu S, Massaro M, Liu H, Lee H, Zhang A, Hamilton DJ, Blanco E.Cells were washed with 1× PBS twice, and the cytoskeleton was stained with Acti-Stain 555 Phalloidin (PHDH1-A, Cytoskeleton, Inc., Denver, CO, USA) for 1 h. Cells were then washed with 1× PBS twice, and nuclei

(3) GxcM-Fbp17/RacC-WASP signaling regulates polarized cortex assembly in migrating cells via Arp2/3Journal of Cell BiologyJune 5, 2023Dong Li, Yihong Yang, Chenglin Lv, Yingjie Wang, Xiaoting Chao, Jiafeng Huang, Shashi P. Singh, Ye Yuan, Chengyu Zhang, Jizhong Lou, et al.mM glycine, and washed with KK2. Cells were then stained with 14 μM Acti-stain 555 phalloidin (cat#PHDH1-A; Cytoskeleton) at room temperature in the dark for 30 min. Images were captured on a Zeiss 880 confocal

(4) Actin filaments accumulated in the nucleus and remain in the vicinity of condensing chromosomes during prophase in zebrafish early embryoBiology OpenMay 15, 2023Haruka Oda, Yuko Sato, Shigehiro A. Kawashima, Yusuke Fujiwara, Máté Pálfy, Edlyn Wu, Nadine L. Vastenhouw, Motomu Kanai, Hiroshi Kimuraμg/ml Hoechst 33342 (Nacalai Tesque, 04929-82) and 20 nM Acti-stain™ 555 phalloidin (Cytoskeleton, PHDH1-A) in PBS containing 0.05% Triton X-100 at 4°C overnight in the dark. Stained embryos were washed three

(5) Chlamydia trachomatis Subverts Alpha-Actinins To Stabilize Its InclusionMicrobiology spectrumFebruary 14, 2023Haines A, Wesolowski J, Paumet F.anti-detyrosinated α-tubulin (rabbit, 48389; Abcam), ActiStain-488 (PHDG1; Cytoskeleton), ActiStain-555 (PHDH1-A; Cytoskeleton), anti-heat shock protein 70 (HSP70) (chicken, SPC-178D; StressMarq), anti-IncA (rabbit;

(6) Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinomaCell death and differentiationFebruary 1, 2023Cao YF, Xie L, Tong BB, Chu MY, Shi WQ, Li X, He JZ, Wang SH, Wu ZY, Deng DX, et al.anti-rabbit IgG (Jackson, 711-545-152, 1:200) and Acti-stain 555 fluorescent phalloidin (Cytoskeleton, PHDH1-A, 1:200) in the dark for 1 h. After washing, cells were counterstained with DAPI (Beyotime, C1005, 1:2000)

(7) Pathogenic tau decreases nuclear tension in cultured neuronsFrontiers in AgingJanuary 23, 2023Claira Sohn, Jiacheng Ma, William J. Ray, Bess Frost(#H-1000-10, Vectorlabs). Latrunculin A-treated cells were incubated with Acti-Stain 555 Phalloidin (#PHDH1-A, Cytoskeleton) prior to DAPI staining. Cells were visualized by confocal microscopy (Zeiss LSM 710 NLO

(8) ZNF185 prevents stress fiber formation through the inhibition of RhoA in endothelial cellsCommunications BiologyJanuary 11, 2023Soichiro Suzuki, Fumiaki Ando, Sae Kitagawa, Yu Hara, Tamami Fujiki, Shintaro Mandai, Koichiro Susa, Takayasu Mori, Eisei Sohara, Tatemitsu Rai, et al.antibodies. The actin cytoskeleton was detected using Acti-stain™ 555 phalloidin (dilution, 1:1000; #PHDH1-A; Cytoskeleton Inc.). The samples were mounted with ProLong™ Glass Antifade Mountant with NucBlue™ (P36981;

(9) Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and miceInternational journal of biological sciencesJanuary 1, 2023Hu M, Bodnar B, Zhang Y, Xie F, Li F, Li S, Zhao J, Zhao R, Gedupoori N, Mo Y, et al.APC anti-mouse CD140a,Biolegend,Cat #: 135907; RRID: AB_2043969 Phalloidin-Fluorescent 555,Cytoskeleton,Cat #: PHDH1-A; RRID: AB_2916284 Donkey anti-mouse Alexa488,Thermo Fisher Scientific,Cat #: A-21202; RRID: AB_141607 Donkey

(10) Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in miceNature communicationsSeptember 28, 2022O'Shea TM, Ao Y, Wang S, Wollenberg AL, Kim JH, Ramos Espinoza RA, Czechanski A, Reinholdt LG, Deming TJ, Sofroniew MV.dihydrochloride (DAPI; 2 ng/mL; Molecular Probes). Acti-stain 555 phalloidin (Cytoskeleton Inc. Cat. # PHDH1-A) staining was performed in conjunction with DAPI nuclei staining using manufacturer’s instructions.

(11) Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discoveryScience advancesSeptember 23, 2022Hiratsuka K, Miyoshi T, Kroll KT, Gupta NR, Valerius MT, Ferrante T, Yamashita M, Lewis JA, Morizane R.added to stain the nucleus. For phalloidin staining, Acti-stain 555 Phalloidin (Cytoskeleton Inc., #PHDH1-A) was used according to the manufacturer’s protocol. Images were taken using a Nikon C1 confocal microscope

(12) Maraviroc inhibits SARS-CoV-2 multiplication and s-protein mediated cell fusion in cell culturebioRxivSeptember 14, 2022Kenneth H. Risner, Katie V. Tieu, Yafei Wang, Allison Bakovic, Farhang Alem, Nishank Bhalla, Steven Nathan, Daniel E. Conway, Paul Macklin, Aarthi Narayanan(ProSci, 3525) was also used to detect expressed S-protein. Actin-stain 555 phalloidin (Cytoskeleton, PHDH1-A) was used to label the actin cytoskeleton. Cells were imaged using a Zeiss LSM 710 Laser Scanning Microscope.

(13) Integrating an aerosolized drug delivery device with conventional static cultures and a dynamic airway barrier microphysiological systemBiomicrofluidicsSeptember 13, 2022Nikita Karra, Joao Fernandes, Emily Jane Swindle, Hywel Morgan60 min at room temperature) before overnight incubation with Acti-stain555-phalloidin (Cytoskeleton, PHDH1-A) and AlexaFluor®488-conjugated mouse anti-human occludin antibody (Life Technologies, Clone OC-3F10)

(14) Recruitment of clathrin to intracellular membranes is sufficient for vesicle formationeLifeJuly 19, 2022Cansu Küey, Méghane Sittewelle, Gabrielle Larocque, Miguel Hernández-González, Stephen J Roylecells,"Park et al., 2013",DNM TKO, "Chemical compound, drug",Acti-stain 555 phalloidin,"Cytoskeleton, Inc.",#PHDH1-A,Final 1:1000 "Chemical compound, drug",Dynamin Inhibitors: Dynole Series Kit,Abcam,ab120474,30 µM "Chemical

(15) The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentationFrontiers in cell and developmental biologyJanuary 1, 2022Music A, Tejeda-González B, Cunha DM, Fischer von Mollard G, Hernández-Pérez S, Mattila PK.anti-phospho-PLCγ2 (1:100; BD Biosciences, 558507) and Acti-Stain 555 Phalloidin (1:150; Cytoskeleton Inc., PHDH1-A) for 1 h in staining buffer. Samples were mounted in FluoroMount-G (Thermo Fisher). Images were acquired

(16) Inflammasome activation leads to cDC1-independent cross-priming of CD8 T cells by epithelial cell-derived antigeneLifeDecember 23, 2021Deets KA, Nichols Doyle R, Rauch I, Vance RE.(Jackson Immunoresearch; 711-545-152) for 60 min at RT, followed by 150 nM Acti-stain 555 phalloidin (Cytoskeleton, Inc; PHDH1-A) and 100 mM DAPI (D1306) for 30 min at RT. Slides were then washed 2× in H2O and sealed under glass

(17) Cross Talk between ARF1 and RhoA Coordinates the Formation of Cytoskeletal Scaffolds during Chlamydia InfectionmBioDecember 21, 2021Haines A, Wesolowski J, Ryan NM, Monteiro-Brás T, Paumet F.anti-detyrosinated α-tubulin (rabbit, 48389; Abcam), ActiStain-488 (PHDG1; Cytoskeleton), Actistain-555 (PHDH1-A; Cytoskeleton), anti-heat shock protein 70 (HSP70) (chicken, SPC-178D; StressMarq), anti-InaC (mouse;

(18) Ensconsin-dependent changes in microtubule organization and LINC complex–dependent changes in nucleus–nucleus interactions result in quantitatively distinct myonuclear positioning defectsMolecular Biology of the CellNovember 1, 2021Mary Ann Collins, L. Alexis Coon, Riya Thomas, Torrey R. Mandigo, Elizabeth Wynn, Eric S. Folkeranti–α-tubulin (1:200; Sigma-Aldrich; T6199) was used. Acti-stain 555 phalloidin (1:400; Cytoskeleton PHDH1-A) and Hoechst 33342 (1 μg/ml) were added with the fluorescent secondary antibody Alexa Fluor 488 donkey

(19) Tolerance and Persistence of Ebola Virus in Primary Cells from Mops condylurus, a Potential Ebola Virus ReservoirVirusesOctober 29, 2021Marcel Bokelmann et al.used as described above. For actin filament staining, a 100 nM solution of Acti-stain 555 Phalloidin (PHDH1-A, Cytoskeleton) was used for 30 min and samples were mounted in ddH2O. 2.6. Viral Genome Analysis To

(20) Neuronal metabotropic glutamate receptor 8 protects against neurodegeneration in CNS inflammationThe Journal of experimental medicineMay 3, 2021Woo MS, Ufer F, Rothammer N, Di Liberto G, Binkle L, Haferkamp U, Sonner JK, Engler JB, Hornig S, Bauer S, et al.visualize neuronal morphology, we used actin-stain 555 phalloidin (1:100; Cytoskeleton; catalog no. PHDH1-A) and actin-stain 670 phalloidin (1:100; Cytoskeleton; catalog no. PHDN1-A). To measure the influence

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12/5/2022Excellent Acti-Stain 555 Phalloidin Matteo MassaroI use this for very good actin staining on my cells (A549, MRC5, human primary cells, MSCs). I reconstitute this in 500 microliters of methanol 100%. I use 7 microliters every 1 ml of working solution... Read Review

Acti-stain™ 555 phalloidin from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Acti-stain™ 555 phalloidin from Cytoskeleton, Inc.

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